Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 44-506G - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-c-Raf (Tyr340, Tyr341) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Storage
- -20°C
Submitted references Inhibition of SHP2-mediated dephosphorylation of Ras suppresses oncogenesis.
CNK1 is a scaffold protein that regulates Src-mediated Raf-1 activation.
Bunda S, Burrell K, Heir P, Zeng L, Alamsahebpour A, Kano Y, Raught B, Zhang ZY, Zadeh G, Ohh M
Nature communications 2015 Nov 30;6:8859
Nature communications 2015 Nov 30;6:8859
CNK1 is a scaffold protein that regulates Src-mediated Raf-1 activation.
Ziogas A, Moelling K, Radziwill G
The Journal of biological chemistry 2005 Jun 24;280(25):24205-11
The Journal of biological chemistry 2005 Jun 24;280(25):24205-11
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Extracts of HEK293 cells overexpressing c-Raf unstimulated (1) or stimulated with active Src (2-5) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer for one hour at room temperature, then incubated with the c-Raf (pYpY 340/341) antibody for two hours at room temperature in a 1% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), non-phosphopeptide corresponding to the phosphopeptide immunogen (3), a generic phosphotyrosine-containing peptide (4), or the phosphopeptide immunogen (5). After washing, the membrane was incubated with goat F (ab’)2 anti-rabbit IgG HRP conjugate (Product # ALI4404) and signals were detected using the Pierce SuperSignal™ method.