PAB0592

antibody from Abnova Corporation

Targeting: RAF1 c-Raf, CRAF, Raf-1

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB0592

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB0592, RRID:AB_982859

Product name

RAF1 (phospho S43) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of RAF1.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Disruption of the Rb--Raf-1 interaction inhibits tumor growth and angiogenesis.

Dasgupta P, Sun J, Wang S, Fusaro G, Betts V, Padmanabhan J, Sebti SM, Chellappan SP
Molecular and cellular biology 2004 Nov;24(21):9527-41

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Raf-1 activation disrupts its binding to keratins during cell stress.

Ku NO, Fu H, Omary MB
The Journal of cell biology 2004 Aug 16;166(4):479-85

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Change in protein flexibility upon complex formation: analysis of Ras-Raf using molecular dynamics and a molecular framework approach.

Gohlke H, Kuhn LA, Case DA
Proteins 2004 Aug 1;56(2):322-37

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

RAF1 (phospho S43) polyclonal antibody (Cat # PAB0592). Western blot analysis of RAF1 expression in human kidney.

Validation comment

Western Blot (Tissue lysate)

Protocol

Protocol

Other assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Dot blot analysis of RAF1 (phospho S43) polyclonal antibody (Cat # PAB0592) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5 ug/mL.

Validation comment

Dot Blot (Peptide)