PAB0593

antibody from Abnova Corporation

Targeting: RAF1 c-Raf, CRAF, Raf-1

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB0593

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB0593, RRID:AB_982860

Product name

RAF1 (phospho S471) polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic phosphopeptide of RAF1.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Disruption of the Rb--Raf-1 interaction inhibits tumor growth and angiogenesis.

Dasgupta P, Sun J, Wang S, Fusaro G, Betts V, Padmanabhan J, Sebti SM, Chellappan SP
Molecular and cellular biology 2004 Nov;24(21):9527-41

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Raf-1 activation disrupts its binding to keratins during cell stress.

Ku NO, Fu H, Omary MB
The Journal of cell biology 2004 Aug 16;166(4):479-85

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Change in protein flexibility upon complex formation: analysis of Ras-Raf using molecular dynamics and a molecular framework approach.

Gohlke H, Kuhn LA, Case DA
Proteins 2004 Aug 1;56(2):322-37

Other assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Dot blot analysis of RAF1 (phospho S471) polyclonal antibody (Cat # PAB0593) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5 ug/mL. P-Pab : phospho-antibody; P-Peptide : phospho-peptide; NP-Peptide : non-phospho-peptide.

Validation comment

Dot Blot (Peptide)