PAB3487

antibody from Abnova Corporation

Targeting: RAF1 c-Raf, CRAF, Raf-1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB3487

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB3487, RRID:AB_1579521

Product name

RAF1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of RAF1.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Regulation of Raf through phosphorylation and N terminus-C terminus interaction.

Chong H, Guan KL
The Journal of biological chemistry 2003 Sep 19;278(38):36269-76

---

Role of Raf in vascular protection from distinct apoptotic stimuli.

Alavi A, Hood JD, Frausto R, Stupack DG, Cheresh DA
Science (New York, N.Y.) 2003 Jul 4;301(5629):94-6

---

Down-regulation of Raf-1 kinase is associated with paclitaxel resistance in human breast cancer MCF-7/Adr cells.

Lee M, Koh WS, Han SS
Cancer letters 2003 Apr 10;193(1):57-64

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of RAF1 polyclonal antibody (Cat # PAB3487) in Jurkat cell lysate. RAF1 (arrow) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Formalin-fixed and paraffin-embedded human cancer tissue reacted with RAF1 polyclonal antibody (Cat # PAB3487) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. BC = breast carcinoma.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)