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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- PAB7870 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB7870, RRID:AB_1712495
- Product name
- RAF1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of RAF1.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Identification of Raf-1 S471 as a novel phosphorylation site critical for Raf-1 and B-Raf kinase activities and for MEK binding.
Serine and tyrosine phosphorylations cooperate in Raf-1, but not B-Raf activation.
Zhu J, Balan V, Bronisz A, Balan K, Sun H, Leicht DT, Luo Z, Qin J, Avruch J, Tzivion G
Molecular biology of the cell 2005 Oct;16(10):4733-44
Molecular biology of the cell 2005 Oct;16(10):4733-44
Serine and tyrosine phosphorylations cooperate in Raf-1, but not B-Raf activation.
Mason CS, Springer CJ, Cooper RG, Superti-Furga G, Marshall CJ, Marais R
The EMBO journal 1999 Apr 15;18(8):2137-48
The EMBO journal 1999 Apr 15;18(8):2137-48
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Western blot of GST fusion protein containing human RAF1. The blot was probed with RAF1 polyclonal antibody (Cat # PAB7870) in the presence (lane 2) or absence (lane 1) of RAF1 (C-terminus) blocking peptide (lane 2).
