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Other assayAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Other assay [1]
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- Product number
- PAB8117 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB8117, RRID:AB_1712523
- Product name
- RAF1 (phospho S259) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of RAF1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Disruption of the Rb--Raf-1 interaction inhibits tumor growth and angiogenesis.
Raf-1 activation disrupts its binding to keratins during cell stress.
Change in protein flexibility upon complex formation: analysis of Ras-Raf using molecular dynamics and a molecular framework approach.
Dasgupta P, Sun J, Wang S, Fusaro G, Betts V, Padmanabhan J, Sebti SM, Chellappan SP
Molecular and cellular biology 2004 Nov;24(21):9527-41
Molecular and cellular biology 2004 Nov;24(21):9527-41
Raf-1 activation disrupts its binding to keratins during cell stress.
Ku NO, Fu H, Omary MB
The Journal of cell biology 2004 Aug 16;166(4):479-85
The Journal of cell biology 2004 Aug 16;166(4):479-85
Change in protein flexibility upon complex formation: analysis of Ras-Raf using molecular dynamics and a molecular framework approach.
Gohlke H, Kuhn LA, Case DA
Proteins 2004 Aug 1;56(2):322-37
Proteins 2004 Aug 1;56(2):322-37
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- Abnova Corporation (provider)
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- Experimental details
- Dot blot analysis of RAF1 (phospho S259) polyclonal antibody (Cat # PAB8117) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.
- Validation comment
- Dot Blot (Peptide)
