Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB8133 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB8133, RRID:AB_1712507
- Product name
- RAF1 (phospho S494) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of RAF1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Disruption of the Rb--Raf-1 interaction inhibits tumor growth and angiogenesis.
Raf-1 activation disrupts its binding to keratins during cell stress.
Change in protein flexibility upon complex formation: analysis of Ras-Raf using molecular dynamics and a molecular framework approach.
Dasgupta P, Sun J, Wang S, Fusaro G, Betts V, Padmanabhan J, Sebti SM, Chellappan SP
Molecular and cellular biology 2004 Nov;24(21):9527-41
Molecular and cellular biology 2004 Nov;24(21):9527-41
Raf-1 activation disrupts its binding to keratins during cell stress.
Ku NO, Fu H, Omary MB
The Journal of cell biology 2004 Aug 16;166(4):479-85
The Journal of cell biology 2004 Aug 16;166(4):479-85
Change in protein flexibility upon complex formation: analysis of Ras-Raf using molecular dynamics and a molecular framework approach.
Gohlke H, Kuhn LA, Case DA
Proteins 2004 Aug 1;56(2):322-37
Proteins 2004 Aug 1;56(2):322-37
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Dot blot analysis of RAF1 (phospho S494) polyclonal antibody (Cat # PAB8133) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.
- Validation comment
- Dot Blot (Peptide)