Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB18217 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB18217, RRID:AB_10716481
- Product name
- RAF1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of RAF1.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Protein kinase C and epidermal growth factor stimulation of Raf1 potentiates adenylyl cyclase type 6 activation in intact cells.
Positive regulation of Raf1-MEK1/2-ERK1/2 signaling by protein serine/threonine phosphatase 2A holoenzymes.
Beazely MA, Alan JK, Watts VJ
Molecular pharmacology 2005 Jan;67(1):250-9
Molecular pharmacology 2005 Jan;67(1):250-9
Positive regulation of Raf1-MEK1/2-ERK1/2 signaling by protein serine/threonine phosphatase 2A holoenzymes.
Adams DG, Coffee RL Jr, Zhang H, Pelech S, Strack S, Wadzinski BE
The Journal of biological chemistry 2005 Dec 30;280(52):42644-54
The Journal of biological chemistry 2005 Dec 30;280(52):42644-54
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from HeLa cells, treated with UV (5 mins), using RAF1 polyclonal antibody (Cat # PAB18217).Peptide "+" means "peptide blocking".
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of HeLa cells, using RAF1 polyclonal antibody (Cat # PAB18217).Peptide "+" means "peptide blocking".
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using RAF1 polyclonal antibody (Cat # PAB18217).Peptide "+" means "peptide blocking".
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)