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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB18335 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB18335, RRID:AB_10714671
- Product name
- RAF1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of RAF1.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.
The protein kinase Pak3 positively regulates Raf-1 activity through phosphorylation of serine 338.
Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, Mann M
Cell 2006 Nov 3;127(3):635-48
Cell 2006 Nov 3;127(3):635-48
The protein kinase Pak3 positively regulates Raf-1 activity through phosphorylation of serine 338.
King AJ, Sun H, Diaz B, Barnard D, Miao W, Bagrodia S, Marshall MS
Nature 1998 Nov 12;396(6707):180-3
Nature 1998 Nov 12;396(6707):180-3
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from 293 cells, treated with PMA (125 ng/mL, 30 mins), using RAF1 polyclonal antibody (Cat # PAB18335).Peptide "+" means "peptide blocking".
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human brain tissue using RAF1 polyclonal antibody (Cat # PAB18335).Peptide "+" means "peptide blocking".
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
