H00005894-M03

antibody from Novus Biologicals

Targeting: RAF1 c-Raf, CRAF, Raf-1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Blocking/Neutralizing (Data presented on provider website)

Proximity ligation assay (Data presented on provider website)

Antibody Data

Product number

H00005894-M03

Provider

Novus Biologicals

Proper citation

Novus Cat#H00005894-M03, RRID:AB_922317

Product name

Mouse Monoclonal Raf-1 Antibody

Antibody type

Monoclonal

Description

IgG purified. RAF1 - v-raf-1 murine leukemia viral oncogene homolog 1 (1H4)

Reactivity

Human, Rat

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Raf-1 Antibody (1H4) [H00005894-M03] - Western blot analysis of RAF1 over-expressed 293 cell line, cotransfected with RAF1 Validated Chimera RNAi or non-transfected control. Blot probed with H00005894-M03. GAPDH (36.1 kDa) used as loading control.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Raf-1 Antibody (1H4) [H00005894-M03] - Analysis of RAF1 expression in transfected 293T cell line by RAF1 monoclonal antibody (M03), clone 1H4. Lane 1: RAF1 transfected lysatE (73.1 KDa). Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Raf-1 Antibody (1H4) [H00005894-M03] - Analysis of RAF1 expression in rat brain.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Raf-1 Antibody (1H4) [H00005894-M03] - Analysis of RAF1 expression in HeLa.