ABIN196619

antibody from antibodies-online

Targeting: RAF1 c-Raf, CRAF, Raf-1

Western blot (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ABIN196619

Provider

antibodies-online

Product name

anti-V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (pSer259) antibody

Antibody type

Polyclonal

Description

Affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Reactivity

Human, Mouse

Host

Rabbit

Epitope

pSer259

Vial size

50 μL

Storage

Store the antibody (in aliquots) at -20°C.

Handling

Avoid repeated freezing and thawing.

References

Submitted references

Regulation of Raf-1 by direct feedback phosphorylation.

Dougherty MK, Müller J, Ritt DA, Zhou M, Zhou XZ, Copeland TD, Conrads TP, Veenstra TD, Lu KP, Morrison DK
Molecular cell 2005 Jan 21;17(2):215-24

---

Novel C-Raf phosphorylation sites: serine 296 and 301 participate in Raf regulation.

Hekman M, Fischer A, Wennogle LP, Wang YK, Campbell SL, Rapp UR
FEBS letters 2005 Jan 17;579(2):464-8

---

Positive and negative regulation of Raf kinase activity and function by phosphorylation.

Chong H, Lee J, Guan KL
The EMBO journal 2001 Jul 16;20(14):3716-27

---

The protein kinase Pak3 positively regulates Raf-1 activity through phosphorylation of serine 338.

King AJ, Sun H, Diaz B, Barnard D, Miao W, Bagrodia S, Marshall MS
Nature 1998 Nov 12;396(6707):180-3

---

Molecular imprinting.

Mosbach K
Trends in biochemical sciences 1994 Jan;19(1):9-14