PA5-97369

antibody from Invitrogen Antibodies

Targeting: RAF1 c-Raf, CRAF, Raf-1

Western blot

ELISA

Antibody data

Submit

Validation data

Product number: PA5-97369 - Provider product page
Provider: Invitrogen Antibodies
Product name: Phospho-c-Raf (Ser338) Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: Positive Samples: NIH/3T3; Cellular Location: Cell membrane, Cytoplasm, Mitochondrion, Nucleus Immunogen sequence: RDSSY
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 μL
Concentration: 0.22 mg/mL
Storage: -20°C, Avoid Freeze/Thaw Cycles

RAF1 protein structure - PA5-97369 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Phospho-c-Raf (Ser338) in paraffin-embedded human placenta. Samples were incubated with Phospho-c-Raf (Ser338) Polyclonal antibody (Product # PA5-97369) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Phospho-c-Raf (Ser338) in paraffin-embedded mouse lung. Samples were incubated with Phospho-c-Raf (Ser338) Polyclonal antibody (Product # PA5-97369) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Phospho-c-Raf (Ser338) in paraffin-embedded rat lung. Samples were incubated with Phospho-c-Raf (Ser338) Polyclonal antibody (Product # PA5-97369) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Phospho-c-Raf (Ser338) in paraffin-embedded human placenta. Samples were incubated with Phospho-c-Raf (Ser338) Polyclonal antibody (Product # PA5-97369) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry analysis of Phospho-c-Raf (Ser338) in paraffin-embedded mouse lung. Samples were incubated with Phospho-c-Raf (Ser338) Polyclonal antibody (Product # PA5-97369) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.