Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA1-26655 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Phospho-c-Raf (Ser621) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA1-26655 detects c-Raf (phospho S621) from human samples. PA1-26655 is expected to cross react with mouse (100% conserved) and rat (100% conserved) due to sequence homology. PA1-26655 has been successfully used in Western blot procedures. The PA1-26655 immunogen is a synthetic phosphopeptide corresponding to a portion of human c-Raf containing serine 621. The sequence is conserved in mouse and rat.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 50 µL
- Concentration
- 0.5 mg/ml
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Nuclear Raf-1 kinase regulates the CXCR5 promoter by associating with NFATc3 to drive retinoic acid-induced leukemic cell differentiation.
The Src-family kinase inhibitor PP2 rescues inducible differentiation events in emergent retinoic acid-resistant myeloblastic leukemia cells.
Geil WM, Yen A
The FEBS journal 2014 Feb;281(4):1170-80
The FEBS journal 2014 Feb;281(4):1170-80
The Src-family kinase inhibitor PP2 rescues inducible differentiation events in emergent retinoic acid-resistant myeloblastic leukemia cells.
Jensen HA, Styskal LE, Tasseff R, Bunaciu RP, Congleton J, Varner JD, Yen A
PloS one 2013;8(3):e58621
PloS one 2013;8(3):e58621
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot antibody-peptide competition and mutant analysis using a Phospho-c-Raf pSer621 polyclonal antibody (Product # PA1-26655). Immunoprecipitates of HEK293 cells overexpressing wild-type c-Raf and stimulated with 50 ng/ml EGF for 3 minutes (1-4) or c-Raf mutant S621A (5) were resolved by SDS-PAGE on a 10% Tris-glycine gel and transferred to PVDF. The membrane was blocked with a 5% BSA-TBST buffer overnight at 4ºC, and incubated with c-Raf (pS621) antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: the phosphopeptide immunogen (1), a generic phosphoserine-containing peptide (2), the non-phosphopeptide corresponding to the phosphopeptide immunogen (3), or no peptide (4, 5). The inset shows the relative amount of total c-Raf protein in the wild-type vs. the S621A mutant extracts via c-Raf pan antibody. The data shows that only the phosphopeptide corresponding to c-Raf (pS621) blocks the antibody signal and that the antibody does not recognize the S621A mutant, demonstrating the specificity of the antibody.