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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- Ab124431 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- Recombinant Raf1 Antibody
- Antibody type
- Recombinant
- Description
- Rabbit anti Human Raf1 Antibody, Recombinant, could be used for WB, ICC, IF and so on.Application:WB: 1/500-1/1000ICC/IF: 1/50-1/200Protein FunctionInvolved in the transduction of mitogenic signals from the cell membrane to the nucleus. Part of the Ras-dependent signaling pathway from receptors to the nucleus. Protects cells from apoptosis mediated by STK3.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
AA 4-20- Vial size
- 100_l,10_l,1ml,50_l
- Concentration
- 1 mg/ml
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Raf1 Antibody (Ab124431) - Western Blot All lanes: Recombinant Raf1 Antibody (Ab124431) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/50000 dilution Predicted band size: 73 kDa Observed band size: 70 kDa Exposure time: 10.0 s
Supportive validation
- Submitted by
- Aladdin Scientific (provider)
- Main image
- Experimental details
- Recombinant Raf1 Antibody (Ab124431) - IHC Immunocytochemistry analysis of Raf1 (green) in MCF7 cells using Recombinant Raf1 Antibody (Ab124431) and cells were counterstained with DAPI (blue).
