PA5-28652

antibody from Invitrogen Antibodies

Targeting: RAF1 c-Raf, CRAF, Raf-1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28652

Provider

Invitrogen Antibodies

Product name

c-Raf Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Recommended positive controls: HeLa (starvation for 24 hr), HeLa (starvation for 24 hr and 200 nM PMA treatment for 30 min). Predicted reactivity: Mouse (98%), Rat (99%), Xenopus laevis (92%), Chicken (97%), Rhesus Monkey (100%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 μL

Concentration

0.55 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of RAF1 in paraformaldehyde-fixed A431 cells using a RAF1 polyclonal antibody (Product # PA5-28652) at a 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

c-Raf Polyclonal Antibody detects Raf1 protein at nucleus on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. C-Raf Polyclonal Antibody (Product # PA5-28652) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of c-Raf was performed in paraffin-embedded human endometrial carcinoma tissue using c-Raf Polyclonal Antibody (Product # PA5-28652) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.