PA5-28652

antibody from Invitrogen Antibodies

Targeting: RAF1 c-Raf, CRAF, Raf-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-28652

Provider

Invitrogen Antibodies

Product name

c-Raf Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HeLa (starvation for 24 hr), HeLa (starvation for 24 hr and 200 nM PMA treatment for 30 min).

Concentration

0.51 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of RAF1 using 30 µg of A431 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a RAF1 polyclonal antibody (Product # PA5-28652) at a dilution of 1:5000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of c-Raf was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 5% SDS-PAGE. Proteins were transferred to a membrane and probed with a c-Raf Polyclonal Antibody (Product # PA5-28652) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of c-Raf was performed by separating 30 µg of untreated (–) and treated (+) HeLa whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a c-Raf Polyclonal Antibody (Product # PA5-28652) at a dilution of 1:1000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of RAF1 in paraformaldehyde-fixed A431 cells using a RAF1 polyclonal antibody (Product # PA5-28652) at a 1:200 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of c-Raf was performed in paraffin-embedded human endometrial carcinoma tissue using c-Raf Polyclonal Antibody (Product # PA5-28652) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

c-Raf Polyclonal Antibody detects Raf1 protein at nucleus on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. C-Raf Polyclonal Antibody (Product # PA5-28652) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.