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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-28652 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- c-Raf Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa (starvation for 24 hr), HeLa (starvation for 24 hr and 200 nM PMA treatment for 30 min). Predicted reactivity: Mouse (98%), Rat (99%), Xenopus laevis (92%), Chicken (97%), Rhesus Monkey (100%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.55 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of RAF1 in paraformaldehyde-fixed A431 cells using a RAF1 polyclonal antibody (Product # PA5-28652) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- c-Raf Polyclonal Antibody detects Raf1 protein at nucleus on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. C-Raf Polyclonal Antibody (Product # PA5-28652) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of c-Raf was performed in paraffin-embedded human endometrial carcinoma tissue using c-Raf Polyclonal Antibody (Product # PA5-28652) at a dilution of 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
