Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- NSB505 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NSB505, RRID:AB_10001865
- Product name
- Rabbit Polyclonal Raf-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.5 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Raf1 [p Ser259] Antibody [NSB505] - Hek293 cell lysates overexpressing c-Raf alone (1) or immunoprecipitates prepared from cells cotransfected with c-Raf, Ras[V12] and Rac1 (2-6) were resolved by SDS-PAGE on 10% polyacrylamide gel and transferred to PVDF, then blocked with 5% BSA-TBST buffer ON at 4C, then incubated with the c-Raf [pSpY338/340] Ab for 2hrs at RT in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), non-phosphopeptide corresponding to the phosphopeptide immunogen (3), generic phosphoserine-containing peptide (4), generic phosphotyrosine-containing peptide (5), or the phosphopeptide immunogen (6). Data show that only the peptide corresponding to c-Raf [pSpY338/340] blocks the Ab signal, demonstrating the specificity of the Ab.