Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- NB300-306 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-306, RRID:AB_10000909
- Product name
- Rabbit Polyclonal Raf-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for the ~74k Raf-1 protein phosphorylated at Ser642.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Raf-1 [p Ser642] Antibody [NB300-306] - Analysis of HeLa lysate showing specific labeling of the ~74 kDa Raf 1 protein phosphorylated at Ser642 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase.