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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- NB300-305 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-305, RRID:AB_10001256
- Product name
- Rabbit Polyclonal Raf-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for the ~74k Raf-1 protein phosphorylated at Ser301. Immunolabeling of the Raf-1 protein band is blocked by lambda-phosphatase treatment
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Raf1 [p Ser301] Antibody [NB300-305] - UV treated human Jurkat cell lysate showing specific immunolabeling of the ~74k Raf-1 protein (Control). The phosphospecificty of this labeling is shown in the second lane (lambda-phosphatase: lambda Ptase). The blot is identical to the control except that it was incubated in lambda Ptase (1200 units for 30 min) before being exposed to the Anti-Ser301< Raf-1. The immunolabeling of Raf-1 is completely eliminated by treatment with lambda Ptase.
