F55019

antibody from NSJ Bioreagents

Targeting: TIMP3 SFD

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F55019

Provider

NSJ Bioreagents

Product name

TIMP3 Antibody

Antibody type

Polyclonal

Description

Purified

Reactivity

Human, Mouse

Host

Rabbit

Vial size

0.08 µl, 0.4 µl

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the TIMP3 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human HepG2 cell lysate with TIMP3 antibody. Expected molecular weight: 24-33 kDa depending on glycosylation level.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of mouse NIH 3T3 cell lysate, in the 1) absence and 2) presence of immunizing peptide, with TIMP3 antibody.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human breast carcinoma tissue with TIMP3 antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human MCF7 cells with TIMP3 antibody; Blue=isotype control, Green= TIMP3 antibody.