PA5-17798

antibody from Invitrogen Antibodies

Targeting: PAK1

Immunoprecipitation

Immunohistochemistry

Antibody data

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Validation data

Product number: PA5-17798 - Provider product page
Provider: Invitrogen Antibodies
Product name: PAK1 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: It is not recommended to aliquot this antibody. This antibody is not cross-reactive with PAK2, PAK3 or other PAK family members.
Reactivity: Human, Mouse, Rat, Guinea Pig, Porcine
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Storage: -20°C

PAK1 protein structure - PA5-17798 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Knockdown of PAK1 was achieved by transfecting Caco-2 with PAK1 specific siRNAs (Silencer® select Product # S10020, S10021). Western blot analysis (Fig. a) was performed using Whole cell extracts from the PAK1 knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with PAK1 Polyclonal Antibody (Product # PA5-17798, 1:1000) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to PAK1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-PAK1 Polyclonal Antibody(Product # PA5-17798) and a 61kDa band corresponding to PAK1 was observed across all tested cell lines and tissues. Whole cell extracts (30 µg lysate) of Jurkat (Lane 1), Caco-2 (Lane 2), MCF7 (Lane 3), U-2 OS (Lane 4), MDA-MB-231 (Lane 5), HEK-293 (Lane 6), Mouse Brain (Lane 7), Rat Brain (Lane 8) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:4000) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).