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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AP3414a - Provider product page
- Provider
- Abcepta
- Proper citation
- Abgent Cat#AP3414a, RRID:AB_2283511
- Product name
- Phospho-PAK1(T423) Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µl
- Concentration
- 0.46 mg/ml
- Storage
- Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Submitted references Thymoquinone-induced conformational changes of PAK1 interrupt prosurvival MEK-ERK signaling in colorectal cancer.
El-Baba C, Mahadevan V, Fahlbusch FB, S SM, Rau TT, Gali-Muhtasib H, Schneider-Stock R
Molecular cancer 2014 Aug 29;13:201
Molecular cancer 2014 Aug 29;13:201
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Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Dot blot analysis of anti-PAK1-pT423 Phospho-specific Pab (Cat.#AP3414a) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.
- Primary Ab dilution
- 1:500
Supportive validation
- Submitted by
- Abcepta (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of Phospho-PAK1-pT423 Antibody(Cat#AP3414a) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
- Primary Ab dilution
- 1:10~50
