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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- GTX111076 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX111076, RRID:AB_10719641
- Product name
- PAK1 antibody [N2C2], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references PAK1 confers chemoresistance and poor outcome in non-small cell lung cancer via β-catenin-mediated stemness.
Chen MJ, Wu DW, Wang YC, Chen CY, Lee H
Scientific reports 2016 Oct 7;6:34933
Scientific reports 2016 Oct 7;6:34933
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: A431 7.5% SDS PAGE GTX111076 diluted at 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: BCL-1 7.5% SDS PAGE GTX111076 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PAK1 antibody [N2C2], Internal detects PAK1 protein by western blot analysis.A. 30 ?g PC-12 whole cell lysate/extract7.5% SDS-PAGEPAK1 antibody [N2C2], Internal (GTX111076) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PAK1 antibody detects PAK1 protein by western blot analysis. Whole cell extracts (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with PAK1 antibody (GTX111076) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed A431, using PAK1(GTX111076) antibody at 1:500 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PAK1 antibody [N2C2], Internal detects PAK1 protein at on HBL435 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded HBL435 xenograft. PAK1 antibody [N2C2], Internal (GTX111076) dilution: 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- PAK1 antibody [N2C2], Internal detects PAK1 protein at cytosol and membrane on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. PAK1 antibody [N2C2], Internal (GTX111076) dilution: 1:500.
