AP32618PU-N

antibody from Acris Antibodies GmbH

Targeting: PAK1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AP32618PU-N

Provider

Acris Antibodies GmbH

Product name

anti PAK1 pSer204

Antibody type

Polyclonal

Antigen

KLH-conjugated, synthetic phospho-peptide corresponding to residues surrounding phosphorylated Ser204 of Human PAK1. Epitope: Ser204 (Central)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western Blot Analysis: Untreated and pervanadate-treated NIH/3T3 cells (Lanes 1 and 2, respectively) were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-PAK1 (Ser204) (1/1,000). Blocking Peptide Analysis: Pervanadate-treated NIH/3T3 lysate was either unblocked, PAK1 Ser204 phosphorylated-peptide blocked, or PAK1 non-phosphorylated peptide blocked (Lanes 3, 4, and 5, respectively) that are that were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-PAK1 (Ser204) (1/1,000). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated PAK1