AP32619PU-N

antibody from Acris Antibodies GmbH

Targeting: PAK1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AP32619PU-N

Provider

Acris Antibodies GmbH

Product name

anti PAK1 pSer199/204

Antibody type

Polyclonal

Antigen

Amino Acids encompassing and including phosphorylated Ser199 and Ser204 of Human PAK1. Epitope: Ser199/204(Central)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Western Blot Analysis: Untreated and pervanadate-treated NIH/3T3 cells (Lanes 1 and 2, respectively) were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-PAK1 (Ser204) (1/1,000). Blocking Peptide Analysis: Pervanadate-treated NIH/3T3 lysate was either unblocked, phosphorylated-peptide blocked, or non-phosphorylated peptide blocked (Lanes 3, 4, and 5, respectively) that are that were resolved by electrophoresis, transferred to PVDF and probed with anti-phospho-PAK1 (Ser204) (1/1,000). Lanes 4 and 5 were blocking peptides were the phosphorylated peptide used as the immunogen and the non-phopshrylated form of the peptide. Proteins were visualized using a Goat anti-Rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Arrow indicates phosphorylated PAK1.