PA5-35408

antibody from Invitrogen Antibodies

Targeting: PAK1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-35408

Provider

Invitrogen Antibodies

Product name

Phospho-PAK1 (Thr84) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with bovine, canine, chicken, non-human primate, rat, sheep and zebrafish based on 100% sequence homology.

Concentration

~0.25 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-PAK1 pThr84 in mouse hippocampal lysate using a Phospho-PAK1 pThr84 polyclonal antibody (Product # PA5-35408). Results show a band at ~68kDa. Phosphospecificity is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that the lysate was incubated in Lambda-Ptase (800 units/1mg protein for 30 min). The immunolabeling is completely eliminated by treatment with lambda-Ptase.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-PAK1 pThr84 in mouse hippocampal lysate using a Phospho-PAK1 pThr84 polyclonal antibody (Product # PA5-35408). Results show a band at ~68kDa. Phosphospecificity is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that the lysate was incubated in Lambda-Ptase (800 units/1mg protein for 30 min). The immunolabeling is completely eliminated by treatment with lambda-Ptase.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot of PAK1 in mouse hippocampal lysate showing specific immunolabeling of a band at ~68 kDa corresponding to Phospho-PAK1 (Thr84) polyclonal antibody (Product # PA5-35408) in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (800 units/1 mg protein for 30 minutes).