TA323822

antibody from OriGene

Targeting: PAK1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA323822

Provider

OriGene

Product name

Anti-PAK1 (Phospho-Thr212) Rabbit Polyclonal Antibody

Antibody type

Polyclonal

Description

Anti-PAK1 (Phospho-Thr212) Rabbit Polyclonal Antibody

Host

Rabbit

Conjugate

Unconjugated

Epitope

PAK1

Isotype

IgG

Antibody clone number

NULL

Vial size

100 µl

Concentration

1mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted band size: 68 kDa . Positive control: 293 cells untreated or treated with forskolin lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: 293 cells untreated with forskolin lysate Lane2: 293 cells treated with forskolin lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted cell location: Cytoplasm; Cell membrane. Positive control: Hela cells. Recommended dilution: 1/ 100-200.The image is immunofluorescence of methanol-fixed Hela cells using PAK1 (Phospho-Thr212) antibody at dilution 1/100. (Original magnification:?—200)

Validation comment

IF

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted cell location: Cytoplasm; Cell membrane. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using PAK1 (Phospho-Thr212) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200)

Validation comment

IHC