Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PPS058 - Provider product page
- Provider
- R&D Systems
- Product name
- Phospho-PAK (T402) Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Human, mouse, non-human primates, and rat ~68-70 kDa PAK-1, 2, 3 phosphorylated at T402. T402 in PAK-2 corresponds to T423 in human PAK-1 in Western blots.
- Reactivity
- Human, Mouse, Rat, Simian
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- For long-term storage,
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Phospho-PAK (T402) by Western Blot. Western blot of rat hippocampal lysate showing specific immunolabeling of the approximately 68-70 kDa PAK protein (Control). The phosphospecificity of this labeling is shown in the second lane ( lambda-phosphatase: lambda PPase). The blot is identical to the control except that it was incubated in lambda PPase (1200 units for 30 minutes) before being exposed to the anti-Phospho-PAK-1, 2, 3 (T402). The immunolabeling of PAK is completely eliminated by treatment with lambda PPase.
