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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- ARG52386 - Provider product page
- Provider
- Arigo
- Product name
- anti-PAK1 phospho (Thr84) antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr84 conjugated to KLH
- Description
- Affinity Purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
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- Arigo (provider)
- Main image
- Experimental details
- Western Blot: mouse hippocampal lysate showing specific immunolabeling of the ~68k PAK1 protein phosphorylated at Thr 84 (control) stained with ARG52386 PAK-1 (phospho Thr84) antibody. Phosphospecificity is shown in the second lane (lambda-phosphatase: λ-Ptase). The blot is identical to the control except that the lysate was incubated in λ-Ptase (800 units/1mg protein for 30 min). The immunolabeling is completely eliminated by treatment with λ-Ptase.
