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Other assayAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Other assay [1]
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- Product number
- PAB0569 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0569, RRID:AB_982786
- Product name
- PAK1 (phospho T212) polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic phosphopeptide of PAK1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Phosphoinositide-dependent kinase 1 and p21-activated protein kinase mediate reactive oxygen species-dependent regulation of platelet-derived growth factor-induced smooth muscle cell migration.
The Down syndrome cell adhesion molecule (DSCAM) interacts with and activates Pak.
Phosphorylation of RhoGDI by Pak1 mediates dissociation of Rac GTPase.
Weber DS, Taniyama Y, Rocic P, Seshiah PN, Dechert MA, Gerthoffer WT, Griendling KK
Circulation research 2004 May 14;94(9):1219-26
Circulation research 2004 May 14;94(9):1219-26
The Down syndrome cell adhesion molecule (DSCAM) interacts with and activates Pak.
Li W, Guan KL
The Journal of biological chemistry 2004 Jul 30;279(31):32824-31
The Journal of biological chemistry 2004 Jul 30;279(31):32824-31
Phosphorylation of RhoGDI by Pak1 mediates dissociation of Rac GTPase.
DerMardirossian C, Schnelzer A, Bokoch GM
Molecular cell 2004 Jul 2;15(1):117-27
Molecular cell 2004 Jul 2;15(1):117-27
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Supportive validation
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- Abnova Corporation (provider)
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- Experimental details
- Dot blot analysis of PAK1 (phospho T212) polyclonal antibody (Cat # PAB0569) on nitrocellulose membrane. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5 ug/mL.
- Validation comment
- Dot Blot (Peptide)
