Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- H00005058-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005058-M02, RRID:AB_546750
- Product name
- Mouse Monoclonal PAK1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PAK1 - p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast)
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Fragile X related protein 1 clusters with ribosomes and messenger RNAs at a subset of dendritic spines in the mouse hippocampus.
Cook D, Sanchez-Carbente Mdel R, Lachance C, Radzioch D, Tremblay S, Khandjian EW, DesGroseillers L, Murai KK
PloS one 2011;6(10):e26120
PloS one 2011;6(10):e26120
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PAK1 Antibody (4D1) [H00005058-M02] - Western Blot analysis of PAK1 expression in HeLa NE ( Cat # L013V3 ).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PAK1 Antibody (4D1) [H00005058-M02] - Analysis of PAK1 expression in transfected 293T cell line by PAK1 monoclonal antibody (M02), clone 4D1.Lane 1: PAK1 transfected lysate (Predicted MW: 49.6 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: PAK1 Antibody (4D1) [H00005058-M02] - Detection limit for recombinant GST tagged PAK1 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: PAK1 Antibody (4D1) [H00005058-M02] - Analysis of monoclonal antibody to PAK1 on HeLa cell. Antibody concentration 35 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PAK1 Antibody (4D1) [H00005058-M02] - Analysis of PAK1 transfected lysate using anti-PAK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PAK1 monoclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PAK1 Antibody (4D1) [H00005058-M02] - Analysis of monoclonal antibody to PAK1 on formalin-fixed paraffin-embedded human stomach. Antibody concentration 3 ug/ml.