Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB2110 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB2110, RRID:AB_1578375
- Product name
- PAK1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PAK1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references The C-terminal kinase domain of the p34cdc2-related PITSLRE protein kinase (p110C) associates with p21-activated kinase 1 and inhibits its activity during anoikis.
Human p21-activated kinase (Pak1) regulates actin organization in mammalian cells.
Human Ste20 homologue hPAK1 links GTPases to the JNK MAP kinase pathway.
Chen S, Yin X, Zhu X, Yan J, Ji S, Chen C, Cai M, Zhang S, Zong H, Hu Y, Yuan Z, Shen Z, Gu J
The Journal of biological chemistry 2003 May 30;278(22):20029-36
The Journal of biological chemistry 2003 May 30;278(22):20029-36
Human p21-activated kinase (Pak1) regulates actin organization in mammalian cells.
Sells MA, Knaus UG, Bagrodia S, Ambrose DM, Bokoch GM, Chernoff J
Current biology : CB 1997 Mar 1;7(3):202-10
Current biology : CB 1997 Mar 1;7(3):202-10
Human Ste20 homologue hPAK1 links GTPases to the JNK MAP kinase pathway.
Brown JL, Stowers L, Baer M, Trejo J, Coughlin S, Chant J
Current biology : CB 1996 May 1;6(5):598-605
Current biology : CB 1996 May 1;6(5):598-605
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PAK1 (arrow) using PAK1 polyclonal antibody (Cat # PAB2110). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PAK1 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with PAK1 polyclonal antibody (Cat # PAB2110) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)