PAB18201

antibody from Abnova Corporation

Targeting: PAK1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB18201

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB18201, RRID:AB_10715740

Product name

PAK1 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of PAK1.

Storage

Store at -20°C.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Phosphorylation of Pak1 by the p35/Cdk5 kinase affects neuronal morphology.

Rashid T, Banerjee M, Nikolic M
The Journal of biological chemistry 2001 Dec 28;276(52):49043-52

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p21-activated kinase (PAK1) is phosphorylated and activated by 3-phosphoinositide-dependent kinase-1 (PDK1).

King CC, Gardiner EM, Zenke FT, Bohl BP, Newton AC, Hemmings BA, Bokoch GM
The Journal of biological chemistry 2000 Dec 29;275(52):41201-9

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of extracts from 293 cells, treated with Etoposide (25 uM, 60 mins), using PAK1 polyclonal antibody (Cat # PAB18201).Peptide "+" means "peptide blocking".

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence analysis of HeLa cells, using PAK1 polyclonal antibody (Cat # PAB18201).Peptide "+" means "peptide blocking".

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using PAK1 polyclonal antibody (Cat # PAB18201).Peptide "+" means "peptide blocking".

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)