Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-12844 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-PAK1 (Thr423) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with bovine, mouse, rat, rabbit and Xenopus based on sequence homology.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 μL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Effects of Different Exercise Training Protocols on Gene Expression of Rac1 and PAK1 in Healthy Rat Fast- and Slow-Type Muscles.
Laine S, Högel H, Ishizu T, Toivanen J, Yli-Karjanmaa M, Grönroos TJ, Rantala J, Mäkelä R, Hannukainen JC, Kalliokoski KK, Heinonen I
Frontiers in physiology 2020;11:584661
Frontiers in physiology 2020;11:584661
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-PAK1 (Thr423) in lysates from Hela cell line, untreated or treated with EGF (1 µg/mL, 10 min). Samples were incubated with Phospho-PAK1 (Thr423) polyclonal antibody (Product # PA5-12844). Phospho-PAK1 (T423) Antibody (upper) or Beta-actin (lower).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Phospho-PAK1 (Thr423) in HeLa cells. Samples were incubated in Phospho-PAK1 (Thr423) polyclonal antibody (Product # PA5-12844) followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Dot blot analysis of Phospho-PAK1 (Thr423). Samples were incubated with Phospho-PAK1 (Thr423) polyclonal antibody (Product # PA5-12844) using a dilution of 0.5 µg/mL. 50 ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed on nitrocellulose membrane.
