Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [3]
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Validation data
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- Product number
- NBP1-85802 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-85802, RRID:AB_11046571
- Product name
- Rabbit Polyclonal PAK1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse, rat PAK1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Initial quantitative proteomic map of 28 mouse tissues using the SILAC mouse.
Geiger T, Velic A, Macek B, Lundberg E, Kampf C, Nagaraj N, Uhlen M, Cox J, Mann M
Molecular & cellular proteomics : MCP 2013 Jun;12(6):1709-22
Molecular & cellular proteomics : MCP 2013 Jun;12(6):1709-22
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PAK1 Antibody [NBP1-85802] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PAK1 Antibody [NBP1-85802] - Analysis in human cell line U-2197.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PAK1 Antibody [NBP1-85802] - Analysis in human cell lines U2OS and MCF-7 using Anti-PAK1 antibody. Corresponding PAK1 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PAK1 Antibody [NBP1-85802] - Staining of human cerebral cortex shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PAK1 Antibody [NBP1-85802] - Staining of human pancreas shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PAK1 Antibody [NBP1-85802] - Staining in human cerebral cortex and pancreas tissues using anti-PAK1 antibody. Corresponding PAK1 RNA-seq data are presented for the same tissues.