PA1-978-A488

antibody from Invitrogen Antibodies

Targeting: PSMB6 DELTA, Y

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-978-A488

Provider

Invitrogen Antibodies

Product name

PSMB6 Polyclonal Antibody, Alexa Fluor™ 488

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Description: This IA6-2 monoclonal antibody reacts with the heavy chain of human immunoglobulin D (IgD). This type I transmembrane protein is co-expressed on the surface of mature naive B cells with membrane IgM. IgD also exists as a soluble form in blood serum. Studies have demonstrated that IgD associates with the B cell receptor and participates in mediating signal transduction upon antigen binding.

Reactivity

Human, Bovine

Host

Rabbit

Conjugate

Green dye

Isotype

IgG

Vial size

50 µL

Concentration

1 mg/mL

Storage

4° C, store in dark, DO NOT FREEZE!

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Proteasome 20S (green) in HeLa cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Proteasome 20S Polyclonal Antibody, AlexaFluor 488 conjugate (Product # PA1-978-A488) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

Conjugate

Green dye