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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00000699-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000699-M01, RRID:AB_425335
- Product name
- BUB1 monoclonal antibody (M01), clone 4C3-4C9
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant BUB1.
- Antigen sequence
MDTPENVLQMLEAHMQSYKGNDPLGVWERYIQWVE
ENFPENKEYLITLLEHLMKEFLDKKKYHNDPRFIS
YCLKFAEYNSDLRQFFEFLYNHGIGTLSSPLYIAW
AGHLEAQGELQHASAVLQRGIQNQAEPREFLQQQY
RLFQTRLTETHLPAQARTSEPLHNVQVLNQMITSK
SNPGNNMACISKNQGSELSGVISSACDKESNMERR
VITISKSEYSVHSSLASKVDVEQVVMYCKEKLIRG
ESEFSFEELRAQKYNQRRKHEQWVNEDRHYMKRKE
ANAFEEQLLKQKMDELHKKLHQVVETSHEDLPASQ
ERSEVNPARMGPSVGSQQELRAPCLPVTYQQTPVN
MEKNPREAPPVVPPLANAISAALVSPATSQSIAPP
VPLKAQTVTDSMFAVASKDAGCVNKSTHEFKPQSG
AEIKEGCETHKVANTSSFHTTPNTSLGMVQATPSK
VQPSPTVHTKEALGFIMNMFQAPTLPDISDDKDEW
QSLDQNEDAFEAQFQKNVRSSGAWGVNKIISSLSS
AFHVFEDGNKENYGLPQPKNKPTGARTFGERSVSR
LPSKPKEEVPHAEEFLDDSTVWGIRCNKTLAPSPK
SPGDFTSAAQLASTPFHKLPVESVHILEDKENVVA
KQCTQATLDSCEENMVVPSRDGKFSPIQEKSPKQA
LSSHMYSASLLRLSQPAAGGVLTCEAELGVEACRL
TDTDAAIAEDPPDAIAGLQAEWMQMSSLGTVDAPN
FIVGNPWDDKLIFKLLSGLSKPVSSYPNTFEWQCK
LPAIKPKTEFQLGSKLVYVHHLLGEGAFAQVYEAT
QGDLNDAKNKQKFVLKVQKPANPWEFYIGTQLMER
LKPSMQHMFMKFYSAHLFQNGSVLVGELYSYGTLL
NAINLYKNTPEKVMPQGLVISFAMRMLYMIEQVHD
CEIIHGDIKPDNFILGNGFLEQDDEDDLSAGLALI
DLGQSIDMKLFPKGTIFTAKCETSGFQCVEMLSNK
PWNYQIDYFGVAATVYCMLFGTYMKVKNEGGECKP
EGLFRRLPHLDMWNEFFHVMLNIPDCHHLPSLDLL
RQKLKKVFQQHYTNKIRALRNRLIVLLLECKRSRK- Isotype
- IgG
- Antibody clone number
- 4C3-4C9
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of BUB1 expression in transfected 293T cell line by BUB1 monoclonal antibody (M01), clone 4C3-4C9.Lane 1: BUB1 transfected lysate(122.4 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged BUB1 is approximately 0.03ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to BUB1 on HeLa cell . [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
