H00000699-D01P

antibody from Abnova Corporation

Targeting: BUB1 BUB1A, BUB1L, hBUB1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00000699-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00000699-D01P, RRID:AB_1571803

Product name

BUB1 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human BUB1 protein.

Antigen sequence

MDTPENVLQMLEAHMQSYKGNDPLGEWERYIQWVE
ENFPENKEYLITLLEHLMKEFLDKKKYHNDPRFIS
YCLKFAEYNSDLHQFFEFLYNHGIGTLSSPLYIAW
AGHLEAQGELQHASAVLQRGIQNQAEPREFLQQQY
RLFQTRLTETHLPAQARTSEPLHNVQVLNQMITSK
SNPGNNMACISKNQGSELSGVISSACDKESNMERR
VITISKSEYSVHSSLASKVDVEQVVMYCKEKLIRG
ESEFSFEELRAQKYNQRRKHEQWVNEDRHYMKRKE
ANAFEEQLLKQKMDELHKKLHQVVETSHEDLPASQ
ERSEVNPARMGPSVGSQQELRAPCLPVTYQQTPVN
MEKNPREAPPVVPPLANAISAALVSPATSQSIAPP
VPLKAQTVTDSMFAVASKDAGCVNKSTHEFKPQSG
AEIKEGCETHKVANTSSFHTTPNTSLGMVQATPSK
VQPSPTVHTKEALGFIMNMFQAPTLPDISDDKDEW
QSLDQNEDAFEAQFQKNVRSSGAWGVNKIISSLSS
AFHVFEDGNKENYGLPQPKNKPTGARTFGERSVSR
LPSKPKEEVPHAEEFLDDSTVWGIRCNKTLAPSPK
SPGDFTSAAQLASTPFHKLPVESVHILEDKENVVA
KQCTQATLDSCEENMVVPSRDGKFSPIQEKSPKQA
LSSHMYSASLLRLSQPAAGGVLTCEAELGVEACRL
TDTDAAIAEDPPDAIAGLQAEWMQMSSLGTVDAPN
FIVGNPWDDKLIFKLLSGLSKPVSSYPNTFEWQCK
LPAIKPKTEFQLGSKLVYVHHLLGEGAFAQVYEAT
QGDLNDAKNKQKFVLKVQKPANPWEFYIGTQLMER
LKPSMQHMFMKFYSAHLFQNGSVLVGELYSYGTLL
NAINLYKNTPEKVMPQGLVISFAMRMLYMIEQVHD
CEIIHGDIKPDNFILGNGFLEQDDEDDLSAGLALI
DLGQSIDMKLFPKGTIFTAKCETSGFQCVEMLSNK
PWNYQIDYFGVAATVYCMLFGTYMKVKNEGGECKP
EGLFRRLPHLDMWNEFFHVMLNIPDCHHLPSLDLL
RQKLKKVFQQHYTNKIRALRNRLIVLLLECKRSRK


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of BUB1 expression in transfected 293T cell line (H00000699-T01) by BUB1 MaxPab polyclonal antibody.Lane 1: BUB1 transfected lysate(122.40 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

BUB1 MaxPab rabbit polyclonal antibody. Western Blot analysis of BUB1 expression in Jurkat.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to BUB1 on HeLa cell. [antibody concentration 10 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between BUB1 and APC. HeLa cells were stained with anti-BUB1 rabbit purified polyclonal 1:1200 and anti-APC mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)