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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB3262 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3262, RRID:AB_1571804
- Product name
- BUB1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of BUB1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references No mutations of the Bub1 gene in human gastric and oral cancer cell lines.
The beta2-adaptin clathrin adaptor interacts with the mitotic checkpoint kinase BubR1.
Genetic and epigenetic inactivation of mitotic checkpoint genes hBUB1 and hBUBR1 and their relationship to survival.
Nakagawa H, Yokozaki H, Oue N, Sugiyama M, Ishikawa T, Tahara E, Yasui W
Oncology reports 2002 Nov-Dec;9(6):1229-32
Oncology reports 2002 Nov-Dec;9(6):1229-32
The beta2-adaptin clathrin adaptor interacts with the mitotic checkpoint kinase BubR1.
Cayrol C, Cougoule C, Wright M
Biochemical and biophysical research communications 2002 Nov 15;298(5):720-30
Biochemical and biophysical research communications 2002 Nov 15;298(5):720-30
Genetic and epigenetic inactivation of mitotic checkpoint genes hBUB1 and hBUBR1 and their relationship to survival.
Shichiri M, Yoshinaga K, Hisatomi H, Sugihara K, Hirata Y
Cancer research 2002 Jan 1;62(1):13-7
Cancer research 2002 Jan 1;62(1):13-7
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of BUB1 polyclonal antibody (Cat # PAB3262) in T-47D cell line lysate. BUB1 (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with BUB1 polyclonal antibody (Cat # PAB3262) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
