RQ5869

antibody from NSJ Bioreagents

Targeting: BUB1 BUB1A, BUB1L, hBUB1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ5869

Provider

NSJ Bioreagents

Product name

Bub1 Antibody

Antibody type

Polyclonal

Description

This highly specific Bub1 antibody is suitable for use in Western blot/Flow cytometry/Direct ELISA applications with mouse and rat samples.

Reactivity

Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 ug

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the Bub1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) mouse testis, 2) rat heart, 3) rat PC-3 and 4) rat RH35 lysate with Bub1 antibody. Predicted molecular weight ~122 kDa.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of mouse HEPA1-6 cells with Bub1 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Bub1 antibody.