NBP1-31584

antibody from Novus Biologicals

Targeting: BUB1 BUB1A, BUB1L, hBUB1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

NBP1-31584

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-31584, RRID:AB_2065925

Product name

Rabbit Polyclonal Bub1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: BUB1 Antibody [NBP1-31584] - Sample (30 ug of whole cell lysate) A: A431 7.5% SDS PAGE, antibody diluted at 1:3000.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Bub1 Antibody [NBP1-31584] - A. 30 ug 293T whole cell lysate/extract 5 % SDS-PAGE BUB1 antibody dilution: 1:1000

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Bub1 Antibody [NBP1-31584] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BUB1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.