Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- LS-C348732 - Provider product page
- Provider
- LSBio
- Product name
- Anti-ZBTB7A / Pokemon Antibody (clone BC17-2.9F) LS-C348732
- Antibody type
- Monoclonal
- Description
- Protein G purified
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- BC17-2.9F
- Vial size
- 100µg
- Storage
- Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles. Store undiluted.
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Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Western Blot analysis using Pokemon Antibody (Clone BC17-2.9F) at 2 ug/ml on MDA-MD-231 breast cancer cell lysate. Secondary antibody was peroxidase conjugated Rabbit anti-Mouse Ig at 1:1000 dilution and developed by enhanced chemiluminescence.
Supportive validation
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Nuclear and cytoplasmic staining of reactive tonsil (Human) is shown on Formalin Fixed Paraffin tissue. The sections were stained at 5 ug/ml with Pokemon Antibody (Clone BC17-2.9F) and peroxidase conjugated anti-Mouse Ig secondary antibody with DAB substrate.
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Nuclear staining of 1643 neuroblastoma cells is shown on Formalin Fixed Paraffin embedded tumor xenograft grown in mice. The sections were stained at 5 ug/ml with Pokemon Antibody (Clone BC17-2.9F) and peroxidase conjugated anti-Mouse Ig secondary antibody with DAB substrate. The high background reflects anti-Mouse secondary antibody also reacting against endogenous Mouse serum immunoglobulins present in the xenografts tissue.