14-3309-80

antibody from Invitrogen Antibodies
Targeting: ZBTB7A DKFZp547O146, FBI-1, LRF, pokemon, ZBTB7, ZNF857A
Provider product page for 14-3309-80
Western blot
Immunohistochemistry

Antibody data

Product number
14-3309-80 - Provider product page
Provider
Invitrogen Antibodies
Product name
Pokemon (LRF) Monoclonal Antibody (13E9), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: This monoclonal antibody reacts with human Leukemia/lymphoma-related factor (LRF). This 65-kDa protein is also known as POKEMON, FBI-1, and Zbtb7a. LRF is a member of the POK family of transcriptional repressors, and is expressed in hematopoietic stem cells, T and B lymphocytes, macrophages, and erythroid cells. This protein is also highly expressed in human non-Hodgkin's lymphoma tissues and diffuse large B-cell lymphomas, demonstrating its prominent role in oncogenic transformation. Studies have demonstrated that LRF is also involved in B cell lineage commitment and development by opposing Notch signaling. Moreover, LRF is critical for erythroid terminal differentiation in the bone marrow by inhibiting Bim-mediated apoptosis. Finally, LRF interacts with Bcl-6 in germinal center B cells. Applications Reported: This 13E9 antibody has been reported for use in immunoblotting (WB) and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This 13E9 antibody has been tested by western blot analysis of the A20 cell line and by immunohistochemical staining of formalin-fixed paraffin embedded human tonsil tissue. This can be used at 1-10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Isotype
IgG
Antibody clone number
13E9
Vial size
25 µg
Concentration
0.5 mg/mL
Storage
4° C
ZBTB7A protein structure - 14-3309-80 shown in red.