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antibody from Invitrogen Antibodies
Targeting: ZBTB7A
DKFZp547O146, FBI-1, LRF, pokemon, ZBTB7, ZNF857A
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- 14-3309-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Pokemon (LRF) Monoclonal Antibody (13E9), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This monoclonal antibody reacts with human Leukemia/lymphoma-related factor (LRF). This 65-kDa protein is also known as POKEMON, FBI-1, and Zbtb7a. LRF is a member of the POK family of transcriptional repressors, and is expressed in hematopoietic stem cells, T and B lymphocytes, macrophages, and erythroid cells. This protein is also highly expressed in human non-Hodgkin's lymphoma tissues and diffuse large B-cell lymphomas, demonstrating its prominent role in oncogenic transformation. Studies have demonstrated that LRF is also involved in B cell lineage commitment and development by opposing Notch signaling. Moreover, LRF is critical for erythroid terminal differentiation in the bone marrow by inhibiting Bim-mediated apoptosis. Finally, LRF interacts with Bcl-6 in germinal center B cells. Applications Reported: This 13E9 antibody has been reported for use in immunoblotting (WB) and immunohistochemical staining of formalin-fixed paraffin embedded tissue sections. Applications Tested: This 13E9 antibody has been tested by western blot analysis of the A20 cell line and by immunohistochemical staining of formalin-fixed paraffin embedded human tonsil tissue. This can be used at 1-10 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Isotype
- IgG
- Antibody clone number
- 13E9
- Vial size
- 100 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references LRF is an essential downstream target of GATA1 in erythroid development and regulates BIM-dependent apoptosis.
Regulation of B versus T lymphoid lineage fate decision by the proto-oncogene LRF.
Maeda T, Ito K, Merghoub T, Poliseno L, Hobbs RM, Wang G, Dong L, Maeda M, Dore LC, Zelent A, Luzzatto L, Teruya-Feldstein J, Weiss MJ, Pandolfi PP
Developmental cell 2009 Oct;17(4):527-40
Developmental cell 2009 Oct;17(4):527-40
Regulation of B versus T lymphoid lineage fate decision by the proto-oncogene LRF.
Maeda T, Merghoub T, Hobbs RM, Dong L, Maeda M, Zakrzewski J, van den Brink MR, Zelent A, Shigematsu H, Akashi K, Teruya-Feldstein J, Cattoretti G, Pandolfi PP
Science (New York, N.Y.) 2007 May 11;316(5826):860-6
Science (New York, N.Y.) 2007 May 11;316(5826):860-6
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoblotting of reduced A20 cell line lysate with 5 µg/mL of Anti-Human Pokemon/LRF Purified. Bands were visualized using Anti-Armenian Hamster IgG HRP.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of formalin-fixed paraffin embedded human tonsil, using 10 µg/mL of Armenian Hamster IgG Isotype Control (Product # 14-4888-81) (left) or Anti-Pokemon Purified (right) followed by Anti-Hamster Biotin (Product # 13-4113-85), and DAB visualization. Nuclei are counterstained with hematoxylin.
