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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-55996 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UBE2E2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: STEAQRVDDS PSTSGGSSDG DQRESVQQEP EREQVQPKKK EGKISSKTAA KLSTSAKRIQ KELAEITLDP PPNCSAGPKG DNIYEWRSTI LGPPGSVYEG GVFFLDITFS PDYPFKPPKV TFRTRIYHC Highest antigen sequence identity to the following orthologs: Mouse - 99%, Rat - 66%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of UBE2E2 in Lane 1: Marker (kDa) 230, 130, 95, 72, 56, 36, 28, 17, 11; Lane 2: Human cell line RT-4; Lane 3: Human cell line U-251MG sp; Lane 4: Human plasma (IgG/HSA depleted); Lane 5: Human liver tissue.; Lane 6: Human tonsil tissue. tissue. Samples were probed using an UBE2E2 Polyclonal Antibody (Product # PA5-55996).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of UBE2E2 in Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells); Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells). Samples were probed using an UBE2E2 Polyclonal Antibody (Product # PA5-55996).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of UBE2E2 in human cell line U-2 OS using an UBE2E2 Polyclonal Antibody (Product # PA5-55996) shows localization to nucleoplasm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of UBE2E2 in human rectal tissue shows strong cytoplasmic positivity in glandular cells. Samples were probed using an UBE2E2 Polyclonal Antibody (Product # PA5-55996).
