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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- MA1-19057 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDK1 Monoclonal Antibody (POH-1)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody will not cross-react with Drosophila, mouse, Xenopus or rat.
- Reactivity
- Human, Bovine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- POH-1
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- 4° C, do not freeze
Submitted references Biological dose estimation of UVA laser microirradiation utilizing charged particle-induced protein foci.
Synthesis and structure-activity relationships of N-6 substituted analogues of 9-hydroxy-4-phenylpyrrolo[3,4-c]carbazole-1,3(2H,6H)-diones as inhibitors of Wee1 and Chk1 checkpoint kinases.
Hela /-CaD undergoes a DNA replication-associated switch in localization from the cytoplasm to the nuclei of endothelial cells/endothelial progenitor cells in human tumor vasculature.
Hela /-CaD undergoes a DNA replication-associated switch in localization from the cytoplasm to the nuclei of endothelial cells/endothelial progenitor cells in human tumor vasculature.
Ixocarpalactone A isolated from the Mexican tomatillo shows potent antiproliferative and apoptotic activity in colon cancer cells.
c-Myc alters the DNA damage-induced G2/M arrest in human mammary epithelial cells.
Splinter J, Jakob B, Lang M, Yano K, Engelhardt J, Hell SW, Chen DJ, Durante M, Taucher-Scholz G
Mutagenesis 2010 May;25(3):289-97
Mutagenesis 2010 May;25(3):289-97
Synthesis and structure-activity relationships of N-6 substituted analogues of 9-hydroxy-4-phenylpyrrolo[3,4-c]carbazole-1,3(2H,6H)-diones as inhibitors of Wee1 and Chk1 checkpoint kinases.
Smaill JB, Baker EN, Booth RJ, Bridges AJ, Dickson JM, Dobrusin EM, Ivanovic I, Kraker AJ, Lee HH, Lunney EA, Ortwine DF, Palmer BD, Quin J 3rd, Squire CJ, Thompson AM, Denny WA
European journal of medicinal chemistry 2008 Jun;43(6):1276-96
European journal of medicinal chemistry 2008 Jun;43(6):1276-96
Hela /-CaD undergoes a DNA replication-associated switch in localization from the cytoplasm to the nuclei of endothelial cells/endothelial progenitor cells in human tumor vasculature.
Zheng PP, Weiden Mv, Sillevis Smitt PA, Luider TM, Kros JM
Cancer biology & therapy 2007 Jun;6(6):886-90
Cancer biology & therapy 2007 Jun;6(6):886-90
Hela /-CaD undergoes a DNA replication-associated switch in localization from the cytoplasm to the nuclei of endothelial cells/endothelial progenitor cells in human tumor vasculature.
Zheng PP, Weiden Mv, Sillevis Smitt PA, Luider TM, Kros JM
Cancer biology & therapy 2007 Jun;6(6):886-90
Cancer biology & therapy 2007 Jun;6(6):886-90
Ixocarpalactone A isolated from the Mexican tomatillo shows potent antiproliferative and apoptotic activity in colon cancer cells.
Choi JK, Murillo G, Su BN, Pezzuto JM, Kinghorn AD, Mehta RG
The FEBS journal 2006 Dec;273(24):5714-23
The FEBS journal 2006 Dec;273(24):5714-23
c-Myc alters the DNA damage-induced G2/M arrest in human mammary epithelial cells.
Sheen JH, Woo JK, Dickson RB
British journal of cancer 2003 Oct 20;89(8):1479-85
British journal of cancer 2003 Oct 20;89(8):1479-85
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blotting analysis of human Cdk1 using mouse Monoclonal antibody POH-1 on lysates of HeLa, MOLT-4, and Jurkat cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2µg/mL of mouse anti-Cdk1 Monoclonal antibody (Product # MA1-19057) followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for Cdk1 protein at approximately 32kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry staining of human thymus (paraffin sections) using anti-Cdk1 monoclonal antibody (Product # MA1-19057).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry staining of human thymus (paraffin sections) using anti-Cdk1 (clone POH-1) Monoclonal antibody (Product # MA1-19057).
