AP7517d

antibody from Abgent

Targeting: CDK1 CDC2, CDC28A

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP7517d

Provider

Abgent

Proper citation

Abgent Cat#AP7517d, RRID:AB_2291295

Product name

CDK1 Antibody (T14)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of CDK1(arrow) using rabbit polyclonal CDK1 Antibody (T14) (Cat.#AP7517d). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDK1 gene (Lane 2) (Origene Technologies).

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with CDK1 Antibody (T14) (Cat.#AP7517d), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:10~50