AP3059a

antibody from Abgent

Targeting: CDK1 CDC2, CDC28A

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP3059a

Provider

Abgent

Proper citation

Abgent Cat#AP3059a, RRID:AB_637596

Product name

Phospho-CDK1(S39) Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.41 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Dot blot analysis of anti-CDK11-S39 Phospho-specific Pab (Cat. #AP3059a) on nitrocellulose membrane. 50ng of nonphospho-peptide or phospho-peptide were adsorbed on their respective dots. Antibody working concentration was 0.5ug per ml.

Primary Ab dilution

1:500

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

The anti-Phospho-CDK1-S39 Pab (Cat. #AP3059a) is used in Western blot for detection in Ramos tissue lysate.

Primary Ab dilution

1:1000

Immunohistochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma."

Primary Ab dilution

1:50~100