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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- NB500-142 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB500-142, RRID:AB_10003405
- Product name
- Rabbit Polyclonal CDC2/CDK1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for the ~38k cdc2 protein phosphorylated at Tyr15. Immunolabeling is blocked by the lambda-phosphatase treatment.
- Reactivity
- Human, Rat, Xenopus
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Control of mitotic exit by PP2A regulation of Cdc25C and Cdk1.
Forester CM, Maddox J, Louis JV, Goris J, Virshup DM
Proceedings of the National Academy of Sciences of the United States of America 2007 Dec 11;104(50):19867-72
Proceedings of the National Academy of Sciences of the United States of America 2007 Dec 11;104(50):19867-72
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Cdc2 [p Tyr15] Antibody [NB500-142] - Western Blot. Western blot of SK-N-MC total cell extracts, treated with hydroxyurea for 22 hours to induce Tyr15 cdc2 phosphorylation. The labeling by the antibody to phospho Tyr15 cdc2 is specifically blocked by the Ser15 phosphopeptide used as antigen. The corresponding non-phosphopeptide did not block the immunolabeling (not shown).
