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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB0434 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB0434, RRID:AB_982419
- Product name
- CDC2 (phospho T161) polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic phosphopeptide (conjugated with KLH) corresponding to residues surrounding T161 of human CDC2.
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 400 μl
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Cyclin C/cdk3 promotes Rb-dependent G0 exit.
Chromosomal mapping of members of the cdc2 family of protein kinases, cdk3, cdk6, PISSLRE, and PITALRE, and a cdk inhibitor, p27Kip1, to regions involved in human cancer.
A family of human cdc2-related protein kinases.
Ren S, Rollins BJ
Cell 2004 Apr 16;117(2):239-51
Cell 2004 Apr 16;117(2):239-51
Chromosomal mapping of members of the cdc2 family of protein kinases, cdk3, cdk6, PISSLRE, and PITALRE, and a cdk inhibitor, p27Kip1, to regions involved in human cancer.
Bullrich F, MacLachlan TK, Sang N, Druck T, Veronese ML, Allen SL, Chiorazzi N, Koff A, Heubner K, Croce CM
Cancer research 1995 Mar 15;55(6):1199-205
Cancer research 1995 Mar 15;55(6):1199-205
A family of human cdc2-related protein kinases.
Meyerson M, Enders GH, Wu CL, Su LK, Gorka C, Nelson C, Harlow E, Tsai LH
The EMBO journal 1992 Aug;11(8):2909-17
The EMBO journal 1992 Aug;11(8):2909-17
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of CDC2 (phospho T161) polyclonal antibody (Cat # PAB0434) in mouse brain tissue lysate (35ug/lane). Phospho-CDC2 (arrow) was detected using the purified polyclonal antibody.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of CDC2 (phospho T161) polyclonal antibody (Cat # PAB0434). HEK-293 cell line lysate (1 ug/lane) either nontransfected (Lane 1) or transiently transfected with the CDC2 gene (Lane 2). CDC2 (phospho T161) polyclonal antibody (arrow) was detected using the purified Pab.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with CDC2 (phospho T161) polyclonal antibody (Cat # PAB0434) which was peroxidase-conjugated to the secondary antibody followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
