200-301-160

antibody from Invitrogen Antibodies

Targeting: CDK1 CDC2, CDC28A

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

200-301-160

Provider

Invitrogen Antibodies

Product name

Cdc2 p34 Monoclonal Antibody (POH-1)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

POH-1

Vial size

100 µg

Concentration

1 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mab anti-Human p34cdc2 antibody (clone POH-1) is shown to detect human p34cdc2by western blot. Detection occurs after 10 µg of a HeLa whole cell lysate is loaded per lane. The blot was incubated with a 1:1,000 dilution of Mab anti-Human p34cdc2at room temperature for 30 min followed by detection using IRDye™800 labeled Goat-a-Mouse IgG [H&L] (610-132-121) diluted 1:5,000. A doublet band corresponding to human p34cdc2is detected at ~34 kDa when compared with known molecular weight standards (not shown). The antibody may be used to detect endogenous human p34cdc2. IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mab anti-Human p34cdc2 antibody was used to detect human p34cdc2by western blot in untreated (control) and drug treated (10 µM genistein) lysates of MCF-7 cells. Very strong detection occurs using a 1:1,000 dilution. Personnel Communication, Xiao He Yang, University of Oklahoma Health Sciences Center.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot of Mouse anti-CDC2 (p34) antibody. Marker: Opal Pre-stained ladder (p/n MB-210-0500). Lane 1: HEK293 lysate (p/n W09-000-365). Lane 2: HeLa Lysate (p/n W09-000-363). Lane 3: MCF-7 Lysate (p/n W09-000-360). Lane 4: Jurkat Lysate (p/n W09-000-370). Lane 5: A549 Lysate (p/n W09-001-372). Lane 6: HL-60 Lysate (p/n W09-001-GL3). Lane 7: Raji Lysate (p/n W09-001-368). Lane 8: NIH/3T3 Lysate (p/n W10-000-358). Load: 35 µg per lane. Primary antibody: CDC2 (p34) antibody at 1:5,000 for overnight at 4°C. Secondary antibody: Peroxidase mouse secondary antibody at 1:30,000 for 60 min at RT. Blocking Buffer: 1% Casein-TTBS for 30 min at RT. Predicted/Observed size: 34 kDa for CDC2 (p34).